The CMC Correlative Microscopy Coverslips are made using a polyester based film with unique reticle patterns. They are specifically designed to allow indentification and location of particular areas of interest with brightfield or fluorescence microscopes and then processing for electron microscopy.
The material of the CMC correlative microscopy coverslips is compatible with cell culture growth and exhibit good adhesion for cell cultures.
Features and benefits of the CMC correlative microscopy coverslips:
|Product||CMC 34A||CMC 35||CMC 71|
|Pattern Size||5 ea 1x1mm||10 x 10mm||10x10mm|
|Divisions||0.1x0.1mm||1 x 1mm||0.5x 0.5mm|
|Coverslip Size||22 x22mm||22x22mm||22x22m|
The CMC correlative microscopy coverslips are not designed to replace the optically superior quartz or glass coverslips; the film itself has a structure which can be visible incertain illumination conditions. The CMC correlative coverslips are designed for correlative microscopy using different imaging techniques. An initial general analysis is performed using light microscopy and then the sample is futher processed for SEM or TEM analysis. The key advantage is that the film is easily cut and punched to allow EM processing.
How to use the CMC correlative microscopy coverslips:
1. Sterilise the CMC correlative coverslip with alcohol, dry and add culture
2. Ensure that the grid of the correlative coverslip is positioned and that the text is readable
3. Record images needed and note the co-ordinates of the region of interest
4.Fix, dehydrate and embed with resin for TEM
5.Place a BEEM capsule, filled with reson onto the coverslip over the selected region of interest
6.Cure resin, detach the correlative coverslip; the footprint of the grid in the resin shows the selected location, trim the block in the selected area and make cuts using an ultra- microtome.
CMC34A - 10 x 10 grids of 0.1mm squares at 5 positions. Indexed 1-10 along the top and A-J down the side. Five positions of 1x1mm each are indexed A-E. Total area 5 x 1mm2